Circulating microRNA Profiles in Acetaminophen Toxicity

对乙酰氨基酚 毒性 医学 丙氨酸转氨酶 小RNA 内科学 药理学 胃肠病学 生物 遗传学 基因
作者
Stephanie Carreiro,James Marvel‐Coen,Rosalind Lee,Brittany Chapman,Victor Ambros
出处
期刊:Journal of Medical Toxicology [Springer Nature]
卷期号:16 (2): 177-187 被引量:4
标识
DOI:10.1007/s13181-019-00739-6
摘要

Acetaminophen toxicity has been associated with elevation of microRNAs. The present study was to evaluate overall microRNA profiles and previously identified microRNAs to differentiate acetaminophen (APAP) toxicity from other causes of transaminase elevation. This was an observational study of adults with presumed acetaminophen toxicity at presentation. Serum samples were collected every 12 hours during hospitalization. Total miRNAs were extracted from plasma and levels of 327 microRNAs were quantified using real-time polymerase chain reaction. A standard measure of miRNA expression (delta-delta cycle threshold) was calculated for each microRNAs. A two-level cluster analysis was performed using a random k-means algorithm. Demographic and clinical characteristics of each cluster were compared using ANOVA, Wilcoxon rank sum, Kruskal-Wallis, and chi-square tests. Performance of specific miRNAs of interest was also evaluated. Twenty-seven subjects were enrolled (21 with a final diagnosis of acetaminophen toxicity), and a total of 61 samples were analyzed. Five clusters were identified, two of which demonstrated clear clinical patterns and included specific elevated miRNAs previously reported to be elevated in APAP toxicity patients. Features associated with clusters 1 and 5 included confirmed acetaminophen toxicity, high peak alanine aminotransferase, and late presentation. Clusters 2–4 contained lower peak microRNAs, lower peak alanine aminotransferase, and heterogeneous clinical characteristics. Severe cases of acetaminophen toxicity showed two distinct patterns of microRNA elevation which were similar to previous work, while less severe cases were difficult to distinguish from non-acetaminophen-associated cases. Further work is needed to incorporate microRNA profiles into the diagnostic algorithm of acetaminophen toxicity.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
mjf111发布了新的文献求助10
25秒前
xiaozhang完成签到 ,获得积分10
27秒前
彩色的冷梅完成签到 ,获得积分10
27秒前
yii完成签到 ,获得积分10
29秒前
zmx完成签到 ,获得积分10
30秒前
钟声完成签到,获得积分0
31秒前
又又完成签到,获得积分10
33秒前
34秒前
john发布了新的文献求助10
40秒前
清脆安南完成签到 ,获得积分10
43秒前
Lexi完成签到 ,获得积分10
45秒前
HSJ完成签到 ,获得积分10
59秒前
jojo665完成签到 ,获得积分10
1分钟前
john完成签到,获得积分10
1分钟前
hsrlbc完成签到,获得积分10
1分钟前
April完成签到,获得积分10
1分钟前
LT完成签到 ,获得积分10
1分钟前
自然的心情完成签到 ,获得积分10
1分钟前
好学者完成签到 ,获得积分10
1分钟前
1分钟前
如泣草芥完成签到,获得积分0
1分钟前
ys1111完成签到 ,获得积分10
1分钟前
笨笨忘幽完成签到,获得积分10
1分钟前
Lesterem完成签到 ,获得积分10
1分钟前
李大宝完成签到 ,获得积分10
1分钟前
灵儿响叮当完成签到,获得积分10
1分钟前
迅速的念芹完成签到 ,获得积分10
1分钟前
伶俐白凝完成签到 ,获得积分10
2分钟前
doclarrin完成签到 ,获得积分10
2分钟前
jewel9完成签到,获得积分10
2分钟前
迷人面包完成签到,获得积分0
2分钟前
e746700020完成签到,获得积分10
2分钟前
复杂觅山完成签到 ,获得积分10
2分钟前
Tong完成签到,获得积分0
2分钟前
CLTTT完成签到,获得积分10
2分钟前
hua完成签到 ,获得积分10
2分钟前
wang完成签到,获得积分10
2分钟前
yaoyh_gc完成签到,获得积分10
2分钟前
俊逸的白梦完成签到 ,获得积分10
2分钟前
2分钟前
高分求助中
Sustainability in Tides Chemistry 2000
Microlepidoptera Palaearctica, Volumes 1 and 3 - 13 (12-Volume Set) [German] 1122
Дружба 友好报 (1957-1958) 1000
The Data Economy: Tools and Applications 1000
Mantiden - Faszinierende Lauerjäger – Buch gebraucht kaufen 700
PraxisRatgeber Mantiden., faszinierende Lauerjäger. – Buch gebraucht kaufe 700
A Dissection Guide & Atlas to the Rabbit 600
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3099800
求助须知:如何正确求助?哪些是违规求助? 2751277
关于积分的说明 7612198
捐赠科研通 2403062
什么是DOI,文献DOI怎么找? 1275162
科研通“疑难数据库(出版商)”最低求助积分说明 616276
版权声明 599053