心力衰竭
兴奋剂
医学
心脏病学
心肌病
内科学
肾上腺素能受体
受体
作者
Sunny Chun Chang,Shuxun Ren,Christoph Rau,Jessica Wang
出处
期刊:Methods in molecular biology
日期:2018-01-01
卷期号:: 207-220
被引量:63
标识
DOI:10.1007/978-1-4939-8597-5_16
摘要
Isoproterenol is used widely for inducing heart failure in mice. Isoproterenol is a nonselective beta-adrenergic agonist. The acute model mimics stress-induced cardiomyopathy. The chronic model mimics advanced heart failure in humans. In this chapter, we describe a protocol that we used to induce heart failure in 100+ strains of inbred mice. Techniques on surgical pump implantation and echocardiography are described in detail. We also discuss the impact of drug dosage, duration, mortality, age, gender, and strain on cardiac remodeling responses. The success of model creation may be assessed by echocardiogram or molecular markers. This chapter may be relevant to those who are interested in using this heart failure model.
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