Inorganic calcium filled bacterial cellulose based hydrogel scaffold: novel biomaterial for bone tissue regeneration

This work emphasizes the structural, physio-chemical characterization and cell biological efficiency analysis of novel inorganic calcium (only calcium phosphate and in combination of calcium phosphate & CaCO3) filled bacterial cellulose (BC) based hydrogel scaffolds. FTIR and TG analysis indicates the presence of BC and inorganic calcium within the hydrogel scaffolds. SEM establishes the porous structures (50–200 µm). Swelling study indicates significant swelling ability in both calcium phosphate filled and calcium phosphate & CaCO3 filled hydrogel scaffolds. Compressive strength (0.24–0.60 MPa) of the calcium filled hydrogel scaffolds are similar like trabecular bone. Significant cell viability (Lep-3) was further noticed until 72,120 and 168 h.