LC‐MS Bioanalysis of Peptides and Polypeptides

An increasing number of research papers and reviews reflect a growing interest in analysis of peptides and polypeptides by liquid chromatography—mass spectrometry (LC-MS). This chapter describes methods, technical approaches and the key challenges for peptide bioanalysis. This includes consideration of peptide physicochemical characteristics for reference standard and biological sample preparation, selection of mass spectrometric detection methods, LC separation techniques, as well as the choice of internal standards. The chapter also provides three example assay protocols to help scientists developing robust peptide bioanalytical methods for their intended research.