磷酸戊糖途径
代谢通量分析
恒化器
波姆裂殖酵母
焊剂(冶金)
柠檬酸循环
生物化学
生物
代谢途径
分泌物
新陈代谢
酵母
糖酵解
化学
酿酒酵母
遗传学
有机化学
细菌
作者
Tobias Klein,Sabrina Lange,Nadine Wilhelm,Matthias Bureik,Tae-Hoon Yang,Elmar Heinzle,Konstantin Schneider
标识
DOI:10.1016/j.ymben.2013.11.001
摘要
Protein secretion in yeast is generally associated with a burden to cellular metabolism. To investigate this metabolic burden in Schizosaccharomyces pombe, we constructed a set of strains secreting the model protein maltase in different amounts. We quantified the influence of protein secretion on the metabolism applying 13C-based metabolic flux analysis in chemostat cultures. Analysis of the macromolecular biomass composition revealed an increase in cellular lipid content at elevated levels of protein secretion and we observed altered metabolic fluxes in the pentose phosphate pathway, the TCA cycle, and around the pyruvate node including mitochondrial NADPH supply. Supplementing acetate to glucose or glycerol minimal media was found to improve protein secretion, accompanied by an increased cellular lipid content and carbon flux through the TCA cycle as well as increased mitochondrial NADPH production. Thus, systematic metabolic analyses can assist in identifying factors limiting protein secretion and in deriving strategies to overcome these limitations.
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