A New Electrochemical Aptasensor for the Analysis of the Vascular Endothelial Growth Factor

The vascular endothelial growth factor (VEGF) is a mitogen and important regulator of angiogenesis by activation the VEGF-receptor tyrosine kinases in endothelial cells. VEGF finds growing interest as a therapeutic target, and its analytical detection has important implications as biomarker for different clinical disorders. Herein, we propose a new strategy to fabricate elegant electrochemical aptasensors, and this strategy has been employed to develop a biosensor for the assay of the concentration of VEGF. In our strategy, an electroactive probe, hemin, is not covalently modified at the end of the oligonucleotide. The method was simple and economical, allowing monitoring of VEGF to low concentrations of 1 nM.