手杖
注意事项
医学
静脉穿刺
检测点注意事项
肝功能检查
丙氨酸转氨酶
丙氨酸转氨酶
天冬氨酸转氨酶
内科学
肝功能
肝损伤
重症监护医学
外科
病理
生物化学
碱性磷酸酶
糖尿病
内分泌学
酶
化学
作者
Nira R. Pollock,Jason P. Rolland,Shailendra Kumar,Patrick Beattie,Sidhartha Jain,Farzad Noubary,Vicki L. Wong,Rebecca A. Pohlmann,Una S. Ryan,George M. Whitesides
出处
期刊:Science Translational Medicine
[American Association for the Advancement of Science (AAAS)]
日期:2012-09-19
卷期号:4 (152)
被引量:281
标识
DOI:10.1126/scitranslmed.3003981
摘要
In developed nations, monitoring for drug-induced liver injury through serial measurements of serum transaminases [aspartate aminotransferase (AST) and alanine aminotransferase (ALT)] in at-risk individuals is the standard of care. Despite the need, monitoring for drug-related hepatotoxicity in resource-limited settings is often limited by expense and logistics, even for patients at highest risk. This article describes the development and clinical testing of a paper-based, multiplexed microfluidic assay designed for rapid, semiquantitative measurement of AST and ALT in a fingerstick specimen. Using 223 clinical specimens obtained by venipuncture and 10 fingerstick specimens from healthy volunteers, we have shown that our assay can, in 15 min, provide visual measurements of AST and ALT in whole blood or serum, which allow the user to place those values into one of three readout "bins" [<3× upper limit of normal (ULN), 3 to 5× ULN, and >5× ULN, corresponding to tuberculosis/HIV treatment guidelines] with >90% accuracy. These data suggest that the ultimate point-of-care fingerstick device will have high impact on patient care in low-resource settings.
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