Effects of temperature, irradiance and pCO2 on the growth and nitrogen utilization of Prorocentrum donghaiense

AME Aquatic Microbial Ecology Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials AME 77:155-166 (2016) - DOI: https://doi.org/10.3354/ame01793 Effects of temperature, irradiance and pCO2 on the growth and nitrogen utilization of Prorocentrum donghaiense Zhangxi Hu1,2, Margaret R. Mulholland3,*, Ning Xu4, Shunshan Duan4 1Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, PR China 2Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, PR China 3Department of Ocean, Earth and Atmospheric Sciences, Old Dominion University, 4600 Elkhorn Avenue, Norfolk, VA 23507-0276, USA 4Research Center of Hydrobiology, Jinan University, 601 W Huangpu Blvd, Guangzhou 510632, PR China *‑Corresponding author: mmulholl@odu.edu ABSTRACT: Environmental factors such as temperature, irradiance, and nitrogen (N) supply affect the growth of Prorocentrum donghaiense, but the interactive effects of these physical factors and the effects of atmospheric CO2 ( pCO2) on growth and N uptake have not been examined. We compared growth kinetics of P. donghaiense grown on 4 different N substrates (nitrate [NO3-], ammonium [NH4+], urea, and glutamic acid [glu]) with respect to temperature, irradiance, and pCO2. Temperature (15 to 30°C) had a positive effect on growth (max. growth rates: 0.17 to 0.65 d-1; optimal temperature: 25 to 30°C); maximum specific growth rates (μmax) declined when cultures were grown at 30°C. P. donghaiense grew well on all 4 N sources, under irradiances ranging from 10 to 180 µmol quanta m-2 s-1. μmax (2.0 ± 0.1 d-1) was observed in cultures growing with NH4+ as the sole N source in the highest irradiance treatment (180 µmol quanta m-2 s-1). These rates were significantly higher than those measured in cultures grown on NO3-, urea, and glu (all ~1.4 d-1). Half-saturation constants (Ks) ranged from 66.5 ± 4.6 to 99.4 ± 6.7 µmol quanta m-2 s-1 for cultures grown with glu or NH4+, respectively, as the sole source of N. Both growth and N uptake rates were higher in cultures grown under elevated pCO2. Our results suggest that P. donghaiense exhibits flexible adaptation for N utilization under broad environmental conditions (temperature, irradiance, pCO2), which may play an important role in the formation and duration of P. donghaiense blooms. KEY WORDS: Prorocentrum donghaiense · Temperature · Irradiance · CO2 · Nitrogen · Growth rate · Nitrogen uptake Full text in pdf format PreviousNextCite this article as: Hu Z, Mulholland MR, Xu N, Duan S (2016) Effects of temperature, irradiance and pCO2 on the growth and nitrogen utilization of Prorocentrum donghaiense. Aquat Microb Ecol 77:155-166. https://doi.org/10.3354/ame01793 Export citation RSS - Facebook - Tweet - linkedIn Cited by Published in AME Vol. 77, No. 3. Online publication date: September 16, 2016 Print ISSN: 0948-3055; Online ISSN: 1616-1564 Copyright © 2016 Inter-Research.