Isolation and structure identification of chemical constituents from seeds of Lepidium apetalum

Objective To isolate the chemical constituents from the seeds of Lepidium apetalum and identify their structures. Methods The powdered seeds of L.apetalum were extracted with 70% ethanol and purified by polyamide,silica gel,RP-C18 and Sephadex LH-20 column chromatography.Chemical structures were identified by MS,1D,and 2D NMR experiments.Results Eleven compounds were isolated from the seeds of L.apetalum,they are quercetin-3-O-[2-O-(6-O-E-sinapoyl)-β-D-glucopymosyl]-β-D-glucopyranoside(1),isorhamnetin-3-O[2-O-(6-O-E-sinapoyl)-β-D-glucopymosyl]-β-D-glucopyranoside(2),quercetin-7-O-β-D-glucopyranoside(3),quercetin-3-O-β-D-glucopyranoside(4),isorhamnetin-3-Oβ-D-glucopyranoside(5),isorhamnetin-7-O-β-D-glucopyranoside(6),kaempferol-7-O-β-D-glucopyranoside(7),quercetin(8),β-sitosterol(9),daucosterol(10),and sucrose(11).Conclusion All these compounds are isolated from the seeds of L.apetalum firstly.Compounds 1 and 2 are isolated from the plants of Lepidium L.and Cruciferae plants for the first time,respectively.