Phase transition monitoring in adipose tissue by multiphoton microscope

In the present study, observations of temperature-mediated phase transitions of the components of adipose tissue were done. Pieces of pork abdominal fat were used as samples of adipose tissue. These samples were heated using a temperature-controlled table and observed using a MPTflex two-photon microscope (JenLab GmbH) with fluorescence lifetime imaging microscopy (FLIM) mode. The analysis of FLIM data was done with a software package “Becker&Hickl”. The developed approach has a great potential for obtaining accurate information on the phase-transition processes occurring during metabolism alterations.