Effect of resveratrol-loaded nanostructured lipid carriers supplementation in cryopreservation medium on post-thawed sperm quality and fertility of roosters

The objective of this study was to assess the effect of resveratrol and resveratrol-loaded nanostructured lipid carriers (NLC) supplementation of semen extender on values for fertility variables of cryopreserved rooster semen. Rooster semen was cryopreserved in modified Beltsville extender containing 0 (control group), resveratrol at 20, 40 and 60 μM and resveratrol-loaded NLC at 20, 40 and 60 μM. After thawing, motility properties, abnormal morphology, viability, membrane functionality, mitochondrial activity, apoptotic status, malondialdehyde (MDA) and antioxidant activities (glutathione peroxidase (GPx), superoxide dismutase (SOD), and total antioxidant capacity (TAC)) and fertility potential (fertility and hatchability rates) were assessed. Using 40 μM resveratrol and resveratrol-loaded NLC improved total motility. The results indicated that sperm fertility and hatching rate, viability and membrane functionality were greater in the 40 μM resveratrol and resveratrol-loaded NLC compared to control group. The percentage of apoptotic spermatozoa in 40 μM resveratrol and resveratrol-loaded NLC group was less compared with the 60 μM resveratrol, 60 resveratrol-loaded NLC and control groups. Mitochondria activity was greater in the 40 μM resveratrol and resveratrol-loaded NLC extender group compared to 20 and 60 μM resveratrol, 60 resveratrol-loaded, and control groups. Also, the 40 μM resveratrol and resveratrol-loaded NLC extender group had a greater TAC and reduced MDA. Morphology and SOD were not affected by dose of resveratrol. The results indicate supplementation of the modified Beltsville extender with 40 μM resveratrol and resveratrol-loaded NLC resulted in a greater quality of frozen-thawed rooster sperm.