Antimicrobial and Antibiofilm Activity of D-amino acids Combined with Nanoparticles against Candida albicans

For the examined acids of D-amino, our findings indicated that D- aspartic (D-asp) acid was of high antifungal potentential, and then D-glycine. Also, the current study aimed to determine the effects of D-amino acids and nanoparticles against Candida albicans adhered cells and biofilms, results illustrated the D-asp concentration of 50 μg /ml caused inhibition as the highest, whereas the least was of D-gly 100 μg /ml of against mature bio-film cell adhesion and. The results proved that the combination of amino acid (D-glycine and D-aspartic acid) and nanoparticles (silver and lithium) at sub MIC concentrations (p≤0.05) inhibited cell adhesion and mature biofilm. Scanning electron microscope (SEM) results in observing the structure consisted of biofilm isolate C.albicans revealed, whereas therapy of D-asp had aneffect on bio-film layers of more losing loss in the layers merged of bio-film and destroyed it. On the other hand, merged lithium along the D-asp effect destroyed cells and caused the bio-film single layer survival.