荧光
分子内力
费斯特共振能量转移
发色团
光化学
猝灭(荧光)
材料科学
转鼓
化学
纳米技术
生物物理学
光学
物理
立体化学
有机化学
亲核细胞
催化作用
生物
作者
Chenxu Yan,Zhiqian Guo,Weijie Chi,Wei Fu,Syed Ali Abbas Abedi,Xiaogang Liu,He Tian,Weihong Zhu
标识
DOI:10.1038/s41467-021-24187-5
摘要
Abstract Intramolecular charge transfer (ICT) is a fundamental mechanism that enables the development of numerous fluorophores and probes for bioimaging and sensing. However, the electron-withdrawing targets (EWTs)-induced fluorescence quenching is a long-standing and unsolved issue in ICT fluorophores, and significantly limits the widespread applicability. Here we report a simple and generalizable structural-modification for completely overturning the intramolecular rotation driving energy, and thus fully reversing the ICT fluorophores’ quenching mode into light-up mode. Specifically, the insertion of an indazole unit into ICT scaffold can fully amplify the intramolecular rotation in donor-indazole-π-acceptor fluorophores (fluorescence OFF), whereas efficiently suppressing the rotation in their EWT-substituted system (fluorescence ON). This molecular strategy is generalizable, yielding a palette of chromophores with fluorescence umpolung that spans visible and near-infrared range. This strategy expands the bio-analytical toolboxes and allows exploiting ICT fluorophores for light-up sensing of EWTs including N -acetyltransferases and nerve agents.
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