克鲁维酵母
生物
酵母
生物化学
分解代谢
基因
人口
琥珀酸
雅罗维亚
氨基丁酸
遗传学
酿酒酵母
新陈代谢
社会学
人口学
受体
作者
Giorgia Perpetuini,Fabrizia Tittarelli,Noemi Battistelli,Giovanna Suzzi,Rosanna Tofalo
摘要
Aims Kluyveromyces marxianus dairy strains were tested for γ-aminobutyric acid (GABA) production. The genes involved in GABA catabolism (UGA1 and UGA2) and anabolism (GAD1) were found in K. marxianus genome. Their relative expression was evaluated with primer designed ad hoc. Methods and Results Strains were grouped on the basis of GAD1 gene sequence. Representative strains for each group were tested for GABA production by high-performance liquid chromatography. All strains produced it at low levels. qRT-PCR showed the absence of a relation between GABA production and GAD1 gene expression. UGA1 and UGA2 genes were not upregulated and low amounts of succinic acid were detected. Conclusions All strains released a low amount of GABA suggesting that probably it was stored within cells. The different behaviour of strains in terms of GABA and succinic acid production as well as gene expression highlighted the genetic and phenotypic biodiversity of this species. Significance and Impact of the Study GABA production and genes involved in its catabolism and anabolism were described in a population of dairy K. marxianus for the first time. The variability observed in terms of genetic and phenotypic biodiversity is important especially to exploit this non-conventional yeast as microbial platform.
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