双金属片
适体
检出限
选择性
电化学
材料科学
线性范围
微球
吸附
纳米技术
化学
化学工程
电极
金属
色谱法
冶金
催化作用
有机化学
物理化学
工程类
生物
遗传学
作者
Kangkai Tian,Yashen Ma,Yongkang Liu,Minghua Wang,Chuanpan Guo,Linghao He,Yingpan Song,Yifan Zhang,Miao Du
标识
DOI:10.1016/j.snb.2019.127199
摘要
This work reported a label-free electrochemical aptasensor based on bimetallic ZnNi metal–organic framework (MOF) microspheres for highly efficient detection of adenosine (AD). Three ZnNi MOFs with different morphologies were prepared by regulating the molar ratio of Zn2+ to Ni2+ (1:1, 1:2, and 1:3) in the preparation processes. The ZnNi MOF (1:2)-based aptasensor displayed excellent sensing performance toward AD compared with ZnNi MOF (1:1) and ZnNi MOF (1:3)-based ones due to its higher electrochemical activity and stronger binding interaction between the aptamer strands and hierarchically nanostructured hollow microspheres. The proposed aptasensor exhibited a low detection limit of 20.32 fg·mL−1 for AD within a wide linear range from 0.0001 to 100 ng·mL−1. This aptasensor also showed excellent selectivity, acceptable reproducibility, high stability, good regenerability, and favorable applicability for AD detection in human serum. The remarkable biosensing performance was primarily attributed to the strong adsorption interactions of Ni2+ to aptamers, special morphology of materials, and the synergistic effect of Zn2+/Ni2+ in MOF matrixes. The rational construction of bimetallic ZnNi MOFs provided a promising sensing platform for sensitive detection of AD and further, showed great potentials in clinic diagnosis.
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