去细胞化
椎间盘
细胞外基质
细胞生物学
组织工程
再生(生物学)
间充质干细胞
生物医学工程
生物相容性
糖胺聚糖
生物材料
干细胞
脚手架
化学
材料科学
解剖
生物
生物化学
医学
有机化学
作者
Lei Yu,Zi‐Jie Sun,Quanchang Tan,Shuang Wang,Weiheng Wang,Xiangqun Yang,Xiao‐Jian Ye
标识
DOI:10.1177/0885328220921328
摘要
Extracellular matrix loss is one of the early manifestations of intervertebral disc degeneration. Stem cell-based tissue engineering creates an appropriate microenvironment for long term cell survival, promising for NP regeneration. We created a decellularized nucleus pulposus hydrogel (DNPH) from fresh bovine nucleus pulposus. Decellularization removed NP cells effectively, while highly preserving their structures and major biochemical components, such as glycosaminoglycan and collagen II. DNPH could be gelled as a uniform grid structure in situ at 37°C for 30 min. Adding adipose marrow-derived mesenchymal stem cells into the hydrogel for three-dimensional culture resulted in good bioactivity and biocompatibility in vitro. Meanwhile, NP-related gene expression significantly increased without the addition of exogenous biological factors. In summary, the thermosensitive and injectable hydrogel, which has low toxicity and inducible differentiation, could serve as a bio-scaffold, bio-carrier, and three-dimensional culture system. Therefore, DNPH has an outstanding potential for intervertebral disc regeneration.
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