染色质免疫沉淀
免疫沉淀
核蛋白
芯片排序
分子生物学
炸薯条
计算机科学
DNA
计算生物学
染色质
细胞生物学
抗体
化学
生物
基因
染色质重塑
遗传学
生物化学
基因表达
电信
发起人
作者
Tae Hoon Kim,Job Dekker
出处
期刊:CSH Protocols
[Cold Spring Harbor Laboratory]
日期:2018-04-01
卷期号:2018 (4): pdb.prot082610-pdb.prot082610
被引量:11
标识
DOI:10.1101/pdb.prot082610
摘要
ChIP is a powerful method for analyzing protein–DNA interactions that occur in vivo. A basic protocol involves immunoprecipitation of the cross-linked nucleoprotein complex and isolating and analyzing the DNA that is associated with the protein of interest. The antibody and chromatin amounts indicated here are for ChIP-chip and ChIP-seq and are ∼10-fold higher than for a standard ChIP assay.
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