Anthocyanin-rich black carrot (Daucus carota ssp. sativus var. atrorubens Alef.) and red cabbage (Brassica oleracea) extracts incorporated biosensor for colorimetric detection of Helicobacter pylori with color image processing

卡罗塔达乌斯 花青素 红卷心菜 甘蓝 化学 幽门螺杆菌 RGB颜色模型 检出限 植物 园艺 色谱法 食品科学 生物 人工智能 计算机科学 遗传学
作者
Gülten Can Sezgin,İsmail Öçsoy
出处
期刊:Brazilian Journal of Microbiology [Springer Nature]
卷期号:54 (2): 897-905 被引量:1
标识
DOI:10.1007/s42770-023-00989-1
摘要

In this work, we developed novel colorimetric biosensors consisting of anthocyanin-rich either black carrot (Daucus carota ssp. sativus var. atrorubens Alef.) or red cabbage (Brassica oleracea) extracts for rapid, sensitive, and economic detection of Helicobacter pylori (H. pylori). We comparatively prepared two test solutions as biosensors including anthocyanin-rich black carrot extract (Anth@BCE) and red cabbage extract (Anth@RCE), both of which fixed to pH 2.5 and investigated their colorimetric responses based on electronic structure and electron density of anthocyanins. We successfully used anthocyanin-rich BCE and RCE as natural pH indicators in detection of H. pylori and introduced their advantages like non-toxicity, easy accessibility, and high stability compared to synthetic indicators. The BCE and RCE tests gave the best color change in the presence of 103 CFU/mL (at 60 min) and 104 CFU/mL (at 75 min) H. pylori suspensions prepared in an artificial gastric fluid. The limit of detection was down to 10 CFU/mL for RCE and BCE tests by increasing incubation time (≥ 5 h). We further made an additional study that color differences in the colorimetric responses observed by naked eyes were supported by digital image processing with RGB (Red Green Blue) and Delta-E (ΔE) analysis. It is confirmed that results evaluated by naked eyes and digital image processing are well consistent with each other. These findings proposed that these colorimetric tests can be implemented to pH dependent detection of various microorganisms and can be effectively transferred from laboratory work to clinics in the near future.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
XYZ完成签到 ,获得积分10
刚刚
pluto应助Fox采纳,获得10
刚刚
费列罗LLL完成签到,获得积分10
刚刚
杨纨成发布了新的文献求助10
1秒前
ylyn发布了新的文献求助10
1秒前
1秒前
华仔应助陌路孤星采纳,获得10
2秒前
修梨发布了新的文献求助10
2秒前
lxl完成签到,获得积分10
2秒前
2秒前
丘比特应助MoleMed采纳,获得10
2秒前
3秒前
未来大牛发布了新的文献求助10
3秒前
华仔应助12采纳,获得10
3秒前
酒酒发布了新的文献求助10
3秒前
FashionBoy应助辛勤的芾采纳,获得10
4秒前
雪白峻熙完成签到,获得积分10
4秒前
wanci应助随便起一个昵称采纳,获得10
4秒前
4秒前
5秒前
zxl完成签到,获得积分10
6秒前
开放念柏发布了新的文献求助10
6秒前
Metbutterly完成签到,获得积分10
6秒前
6秒前
xl完成签到,获得积分10
6秒前
1232112发布了新的文献求助10
7秒前
华仔应助明理念桃采纳,获得10
7秒前
水木年华发布了新的文献求助10
7秒前
之一完成签到 ,获得积分10
7秒前
笨笨丹烟完成签到,获得积分10
7秒前
李健应助修梨采纳,获得10
8秒前
8秒前
桐桐应助愉快的钢铁侠采纳,获得10
8秒前
一只耳完成签到,获得积分10
9秒前
00发布了新的文献求助10
9秒前
未来大牛完成签到,获得积分10
9秒前
9秒前
笨笨丹烟发布了新的文献求助10
9秒前
SciGPT应助小七采纳,获得10
10秒前
高分求助中
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
Effective Learning and Mental Wellbeing 300
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3974358
求助须知:如何正确求助?哪些是违规求助? 3518706
关于积分的说明 11195521
捐赠科研通 3254897
什么是DOI,文献DOI怎么找? 1797614
邀请新用户注册赠送积分活动 877011
科研通“疑难数据库(出版商)”最低求助积分说明 806128