A Dual-Mode Colorimetric and Fluorescence Biosensor Based on a Nucleic Acid Multiplexing Platform for the Detection of Listeria monocytogenes

Listeria monocytogenes (L. monocytogenes) is one of the most prevalent threats, capable of inducing diverse illnesses and presenting a serious threat to public health. Herein, we demonstrate a novel dual-mode colorimetric/fluorescence biosensor based on the exponential amplification reaction and strand displacement reactions (EASDR), which has multiplexing capability that significantly promotes the anchoring and trapping of Pt nanoparticles (Pt NPs) and fluorescent dyes for sensitive detection of Listeria monocytogenes (L. monocytogenes). The method works by targeting specific bacteria with aptamers and promoting repeated EASDR to affect the immobilization of Pt NPs and fluorescent dyes in the orifice plate, which could produce changes in fluorescence and colorimetric signals. The assay achieves a detection limit of 38 CFU/mL in colorimetric and 10 CFU/mL in fluorescence. Furthermore, the developed biosensor can be applied to the analysis of raw food samples (milk), and good recoveries were obtained in spiked food samples. In summary, the dual-mode biosensor improves the accuracy of detection by simultaneously outputting two signals and shows great potential in the specific identification and detection of foodborne pathogens.