材料科学
丝素
生物相容性
免疫原性
热稳定性
乙型肝炎表面抗原
热稳定性
丝绸
纳米技术
化学工程
复合材料
生物物理学
病毒学
化学
生物化学
乙型肝炎病毒
酶
病毒
免疫系统
冶金
工程类
生物
免疫学
作者
J.L. Zhang,Kai Wang,Shiyao Xu,Linlin Chen,Haiquan Gu,Yujie Yang,Qi Zhao,Yurou Huo,Bo Li,Yufei Wang,Yubiao Xie,Nan Li,Jiali Zhang,Yao‐Hua Zhang,Qianxue Li
标识
DOI:10.1021/acsami.3c16358
摘要
Vaccines are widely regarded as one of the most effective weapons in the fight against infectious diseases. Currently, vaccines must be stored and transported at low temperatures as high temperatures can lead to a loss of vaccine conformation and reduced therapeutic efficacy. Metal–organic frameworks (MOFs), such as zeolitic imidazole framework-8 (ZIF-8), are a new class of hybrid materials with large specific surface areas, high loading rates, and good biocompatibility and are successful systems for vaccine delivery and protection. Silk fibroin (SF) has a good biocompatibility and thermal stability. In this study, the hepatitis B surface antigen (HBsAg) was successfully encapsulated in ZIF-8 to form HBsAg@ZIF-8 (HZ) using a one-step shake and one-pot shake method. Subsequently, the SF coating modifies HZ through hydrophobic interactions to form HBsAg/SF@ZIF-8 (HSZ), which enhanced the thermal stability and immunogenicity of HBsAg. Compared to free HBsAg, HZ and HSZ improved the thermostability of HBsAg, promoted the antigen uptake and lysosomal escape, stimulated dendritic cell maturation and cytokine secretion, formed an antigen reservoir to promote antibody production, and activated CD4+ T and CD8+ T cells to enhance memory T-cell production. Importantly, HSZ induced a strong immune response even after 14 days of storage at 25 °C. Furthermore, the nanoparticles prepared by the one-step shake method exhibited superior properties compared to those prepared by the one-pot shake method. This study highlights the importance of SF-coated ZIF-8, which holds promise for investigating thermostable vaccines and breaking the vaccine cold chain.
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