纳米传感器
大肠杆菌
肺炎克雷伯菌
抗菌剂
尿
抗生素
微生物学
纳米技术
生物
材料科学
生物化学
基因
作者
Ruijia Huang,Xiaoqing Cai,Jihui Du,Jie Lian,Ping Hui,Minxuan Gu,Feng Li,Jidong Wang,Wenwen Chen
出处
期刊:ACS Nano
[American Chemical Society]
日期:2022-10-25
卷期号:16 (11): 19229-19239
被引量:13
标识
DOI:10.1021/acsnano.2c08532
摘要
Delayed use of appropriate antibiotics for superbugs, particularly for extended-spectrum β-lactamase (ESBL)-producing Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pn), has caused extensive morbidity and mortality worldwide. Therefore, rapid and on-site antimicrobial susceptibility testing (AST) is urgently required. Unfortunately, currently, no phenotypic AST can realize a sample-to-answer result within 2 h directly from a clinical sample and without using laboratory equipment or customized devices. Inspired by observing that E. coli and K. pn can rapidly catalyze H2O2, we developed a plasmonic nanosensor that responds to the proliferation of bacteria for realizing rapid AST. The results can be determined with the naked eye, digitized using a smartphone, and validated using ultraviolet–visible spectrometry. Our assay achieved superb area under the curves of 0.9752 and 1 in a receiver operating characteristic analysis directly obtained from uncultured clinical urine samples infected by E. coli and K. pn, respectively. The entire process from sample collection to analysis takes 100 min for E. coli and 85 min for K. pn detection. Our platform provides a practical approach for performing on-site AST in clinics to improve the survival of patients. It releases the burden of superbugs and avoids the abuse of antibiotics.
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