胞苷
G-四倍体
RNA剪接
核糖核酸
DNA
信使核糖核酸
抄写(语言学)
端粒
化学
翻译(生物学)
细胞生物学
DNA损伤
生物
基因
生物化学
酶
语言学
哲学
作者
Xucong Teng,Difei Hu,Yicong Dai,Haitao Jing,Wenxuan Hu,Qiushuang Zhang,Na Zhang,Jinghong Li
标识
DOI:10.1002/anie.202407353
摘要
To explore the mechanisms and therapeutic strategies for G‐quadruplex (G4) mediated diseases, it is crucial to manipulate and intervene in intracellular G4 structures using small molecular tools. While hundreds of G4 stabilizers have been developed, there is a significant gap in the availability of G4 unwinding agents. Here, we propose a strategy to disrupt G‐quadruplexes by forming G‐C hydrogen bonds with chemically modified cytidine trimers. We validated a good G4 unwinder, the 2'‐F cytidine trimer (2'‐F C3). 2'‐F C3 does not inhibit cell growth nor cause severe DNA damage at a concentration below 10 μM. Moreover, 2'‐F C3 does not affect gene transcription nor RNA splicing, while it significantly enhances the translation of G4‐containing mRNA and upregulates RNA splicing, RNA processing and cell cycle pathways. The discovery of this G4 unwinder provides a functional tool for the chemical modulation of G4s in living cells.
科研通智能强力驱动
Strongly Powered by AbleSci AI