Transient receptor potential melastatin 3 dysfunction in post COVID-19 condition and myalgic encephalomyelitis/chronic fatigue syndrome patients

脑脊髓炎 慢性疲劳综合征 医学 内科学 免疫学 多发性硬化
作者
Etianne Martini Sasso,Katsuhiko Muraki,Natalie Eaton-Fitch,Peter Smith,Olivia Ly Lesslar,Gary Deed,Sonya Marshall-Gradisnik
出处
期刊:Molecular Medicine [BioMed Central]
卷期号:28 (1) 被引量:1
标识
DOI:10.1186/s10020-022-00528-y
摘要

Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a severe multisystemic condition associated with post-infectious onset, impaired natural killer (NK) cell cytotoxicity and impaired ion channel function, namely Transient Receptor Potential Melastatin 3 (TRPM3). Long-term effects of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus has resulted in neurocognitive, immunological, gastrointestinal, and cardiovascular manifestations recently recognised as post coronavirus disease 2019 (COVID-19) condition. The symptomatology of ME/CFS overlaps significantly with post COVID-19; therefore, this research aimed to investigate TRPM3 ion channel function in post COVID-19 condition patients.Whole-cell patch-clamp technique was used to measure TRPM3 ion channel activity in isolated NK cells of N = 5 ME/CFS patients, N = 5 post COVID-19 patients, and N = 5 healthy controls (HC). The TRPM3 agonist, pregnenolone sulfate (PregS) was used to activate TRPM3 function, while ononetin was used as a TRPM3 antagonist.As reported in previous research, PregS-induced TRPM3 currents were significantly reduced in ME/CFS patients compared with HC (p = 0.0048). PregS-induced TRPM3 amplitude was significantly reduced in post COVID-19 condition compared with HC (p = 0.0039). Importantly, no significant difference was reported in ME/CFS patients compared with post COVID-19 condition as PregS-induced TRPM3 currents of post COVID-19 condition patients were similar of ME/CFS patients currents (p > 0.9999). Isolated NK cells from post COVID-19 condition and ME/CFS patients were resistant to ononetin and differed significantly with HC (p < 0.0001).The results of this investigation suggest that post COVID-19 condition patients may have impaired TRPM3 ion channel function and provide further evidence regarding the similarities between post COVID-19 condition and ME/CFS. Impaired TRPM3 channel activity in post COVID-19 condition patients suggest impaired ion mobilisation which may consequently impede cell function resulting in chronic post-infectious symptoms. Further investigation into TRPM3 function may elucidate the pathomechanism, provide a diagnostic and therapeutic target for post COVID-19 condition patients and commonalities with ME/CFS patients.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
勤奋的如松完成签到,获得积分0
4秒前
粥可温完成签到,获得积分10
6秒前
曾珍发布了新的文献求助10
7秒前
8秒前
hzh完成签到 ,获得积分10
9秒前
gg发布了新的文献求助10
9秒前
勤劳滑板完成签到 ,获得积分10
9秒前
Jerry完成签到,获得积分10
10秒前
MrLiu完成签到,获得积分10
11秒前
冷傲博完成签到,获得积分10
11秒前
jeff完成签到,获得积分10
11秒前
LHZ完成签到,获得积分10
11秒前
所所应助时尚俊驰采纳,获得10
12秒前
影子芳香完成签到 ,获得积分10
13秒前
14秒前
14秒前
不必要再讨论适合与否完成签到,获得积分0
15秒前
无情夏寒完成签到 ,获得积分10
16秒前
慕青应助马士全采纳,获得10
17秒前
xuzj应助科研通管家采纳,获得10
17秒前
Rubby应助科研通管家采纳,获得30
18秒前
SciGPT应助科研通管家采纳,获得10
18秒前
18秒前
18秒前
shiizii应助科研通管家采纳,获得10
18秒前
18秒前
18秒前
18秒前
ludong_0应助科研通管家采纳,获得10
18秒前
YeeYee发布了新的文献求助10
18秒前
冷酷的松思完成签到,获得积分10
18秒前
zgt01发布了新的文献求助10
19秒前
zhang完成签到,获得积分10
19秒前
江中完成签到 ,获得积分10
21秒前
21秒前
阿玖完成签到 ,获得积分10
22秒前
jiaolulu发布了新的文献求助10
24秒前
踏雪飞鸿完成签到,获得积分10
25秒前
hannah完成签到,获得积分10
25秒前
songvv发布了新的文献求助10
26秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds.Vol2 1100
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038201
求助须知:如何正确求助?哪些是违规求助? 3575940
关于积分的说明 11373987
捐赠科研通 3305747
什么是DOI,文献DOI怎么找? 1819274
邀请新用户注册赠送积分活动 892662
科研通“疑难数据库(出版商)”最低求助积分说明 815022