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Increased expression of YAP1 in prostate cancer correlates with extraprostatic extension

雅普1 河马信号通路 前列腺癌 免疫组织化学 癌症研究 免疫印迹 癌变 生物 癌症 病理 分子生物学 医学 转录因子 细胞生物学 信号转导 基因 遗传学
作者
Kisaayak Collak Filiz,Ümmühan Demir,Ozkanli Seyma,Esra Kürüm,Engin Zerk Pinar
出处
期刊:Cancer biology and medicine [Cancer Biology and Medicine]
卷期号:14 (4): 405-405 被引量:34
标识
DOI:10.20892/j.issn.2095-3941.2017.0083
摘要

Objective:

Yes associated protein 1 (YAP1) is a member of the Hippo pathway, acting as a transcriptional coactivator. To elucidate the role of YAP1 and phosphorylated (p)YAP1 in prostate cancer (PCa) tumorigenesis, we investigated their expression in clinical samples of PCa and cell lines.

Methods:

Fifty-four tumor, adjacent nontumor, and prostate intraepithelial neoplasia (PIN) tissues from patients with PCa after radical prostatectomy were selected from a retrospective cohort and studied using immunohistochemistry (IHC). Protein and mRNA expression levels of YAP1 were evaluated by Western blot analysis and quantitative real-time reverse transcription PCR, respectively, in cancer cell lines. Publicly available gene expression datasets were downloaded to analyze YAP1 mRNA and protein levels in PCa tissue samples.

Results:

IHC analysis of PCa tissues revealed that YAP1 staining intensities were moderate to weak in the nucleus and cytoplasm of tumor cells, whereas adjacent normal epithelia showed strong staining. We observed that benign prostates were characterized by higher expression levels of both nuclear (P=0.004) and cytosolic (P=0.005) YAP1. pYAP1 staining was weak in the cytoplasm and absent in the nucleus of all the tissues investigated. YAP1 expression was an indicator of extraprostatic extension (EPE). The level of YAP1 was negatively correlated with the level of the androgen receptor (AR) in The Cancer Genome Atlas dataset and Western blot analysis of cell lines.

Conclusions:

Our study suggested that YAP1 expression is heterogeneous in PCa tissue samples; therefore, YAP1 might play different roles in different aspects of PCa progression. This might involve AR–YAP1 interplay in PCa.
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