藤黄固氮菌
生物高聚物
固氮菌科
化学
食品科学
聚-3-羟基丁酸酯
聚合物
分子质量
生物化学
有机化学
氮气
酶
固氮酶
固氮
作者
Modesto Millán,Miriam Salazar,Daniel Segura,Tania Castillo,A Mendoza Diaz,Carlos Peña
标识
DOI:10.1016/j.jbiotec.2017.08.016
摘要
Poly-3-hydroxybutyrate (P3HB) is a biopolymer produced by Azotobacter vinelandii. The physicochemical properties and applications of P3HB are strongly influenced by its weight-average molecular mass (Mw), and in A. vinelandii, it could be influenced by the culture conditions. The aim of this study was to evaluate the effect of the P3HB content of the inoculum on the Mw of the polymer produced by A. vinelandii OP in bioreactor cultures. A. vinelandii cells containing 20, 50 and 70% of P3HB were used as inoculum. The P3HB content in the inoculum affected the volumetric P3HB productivity (qP3HB) and the Mw of P3HB. Those cultures inoculated with cells containing 20% of P3HB, achieved the highest qP3HB (0.17 ± 0.018 gP3HB L−1 h−1); whereas a P3HB content of 70% was reflected as a low qP3HB (0.021 ± 0.002 gP3HB L−1 h−1). On the other hand, using an inoculum with 70% of polymer content, the Mw of the biopolymer remained stable at values close to 3200 kDa; whereas, when an inoculum with 20% of P3HB was used, the Mw decreased drastically during early stages of cultivation. These results show that manipulating the P3HB content of the inoculum is possible to produce biopolymers with a suitable Mw.
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