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Scalable Lentiviral Vector Production Using Stable HEK293SF Producer Cell Lines

生物 载体(分子生物学) 可扩展性 生产(经济) 病毒载体 计算生物学 细胞培养 病毒学 细胞生物学 计算机科学 遗传学 重组DNA 基因 操作系统 宏观经济学 经济
作者
Aziza Manceur,Howard S. Kim,Vanja Misic,Nadejda Andreev,July Dorion-Thibaudeau,Stéphane Lanthier,Alice Bernier,Sonia Tremblay,Anne-Marie Gélinas,Sophie Broussau,Rénald Gilbert,Sven Ansorge
出处
期刊:Human Gene Therapy Methods [Mary Ann Liebert, Inc.]
卷期号:28 (6): 330-339 被引量:92
标识
DOI:10.1089/hgtb.2017.086
摘要

Human Gene Therapy MethodsVol. 28, No. 6 Research ArticlesOpen AccessScalable Lentiviral Vector Production Using Stable HEK293SF Producer Cell LinesAziza P. Manceur, Howard Kim, Vanja Misic, Nadejda Andreev, July Dorion-Thibaudeau, Stéphane Lanthier, Alice Bernier, Sonia Tremblay, Anne-Marie Gélinas, Sophie Broussau, Rénald Gilbert, and Sven AnsorgeAziza P. ManceurHuman Health Therapeutics, National Research Council Canada, Montreal, CanadaSearch for more papers by this author, Howard KimBridGE, Centre for Commercialization of Regenerative Medicine, Toronto, Canada.Search for more papers by this author, Vanja MisicBridGE, Centre for Commercialization of Regenerative Medicine, Toronto, Canada.Search for more papers by this author, Nadejda AndreevBridGE, Centre for Commercialization of Regenerative Medicine, Toronto, Canada.Search for more papers by this author, July Dorion-ThibaudeauHuman Health Therapeutics, National Research Council Canada, Montreal, CanadaSearch for more papers by this author, Stéphane LanthierHuman Health Therapeutics, National Research Council Canada, Montreal, CanadaSearch for more papers by this author, Alice BernierHuman Health Therapeutics, National Research Council Canada, Montreal, CanadaSearch for more papers by this author, Sonia TremblayHuman Health Therapeutics, National Research Council Canada, Montreal, CanadaSearch for more papers by this author, Anne-Marie GélinasHuman Health Therapeutics, National Research Council Canada, Montreal, CanadaSearch for more papers by this author, Sophie BroussauHuman Health Therapeutics, National Research Council Canada, Montreal, CanadaSearch for more papers by this author, Rénald GilbertHuman Health Therapeutics, National Research Council Canada, Montreal, CanadaSearch for more papers by this author, and Sven AnsorgeHuman Health Therapeutics, National Research Council Canada, Montreal, CanadaSearch for more papers by this authorPublished Online:1 Dec 2017https://doi.org/10.1089/hgtb.2017.086AboutSectionsView articleSupplemental MaterialPDF/EPUBView Supplemental Data Permissions & CitationsPermissionsDownload CitationsTrack CitationsAdd to favorites Back To Publication ShareShare onFacebookTwitterLinked InRedditEmail View articleFiguresReferencesRelatedDetailsCited byPackaging cells for lentiviral vectors generated using the cumate and coumermycin gene induction systems and nanowell single-cell cloningMolecular Therapy - Methods & Clinical Development, Vol. 29Process intensification strategies toward cell culture‐based high‐yield production of a fusogenic oncolytic virus21 February 2023 | Biotechnology and Bioengineering, Vol. 35Enhancing recombinant protein and viral vector production in mammalian cells by targeting the YTHDF readers of N6 ‐methyladenosine in mRNA5 February 2023 | Biotechnology Journal, Vol. 592Process for production of chimeric antigen receptor-transducing lentivirus particles using infection with replicon particles containing self-replicating RNAsBiochemical Engineering Journal, Vol. 191Development of stable HEK293T cell pools expressing CSFV E2 protein: A potential antigen expression platformVaccine, Vol. 41, No. 9Incubation Temperature and Period During Denarase Treatment and Microfiltration Affect the Yield of Recombinant Adenoviral Vectors During Downstream Processing30 November 2022 | Molecular 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38 kDa protein14 September 2021 | World Journal of Microbiology and Biotechnology, Vol. 37, No. 10The Engineered MARCH8-Resistant Vesicular Stomatitis Virus Glycoprotein Enhances Lentiviral Vector Transduction Fengwen Xu, Xiaoman Liu, Di Zhang, Fei Zhao, Zhangling Fan, Siqi Hu, Shan Mei, Yu Huang, Hong Sun, Liang Wei, Li Guo, Jianwei Wang, Shan Cen, Chen Liang, and Fei Guo23 September 2021 | Human Gene Therapy, Vol. 32, No. 17-18Lentiviral Vectors for T Cell Engineering: Clinical Applications, Bioprocessing and Future Perspectives2 August 2021 | Viruses, Vol. 13, No. 8The transformative potential of HSC gene therapy as a genetic medicine26 May 2021 | Gene Therapy, Vol. 11Short Time to Market and Forward Planning Will Enable Cell Therapies to Deliver R&D Pipeline Value Delfi Krishna, Laure Rittié, Hoang Tran, Xuan Zheng, Chia-en Chen-Rogers, Amanda McGillivray, Timothy Clay, Amol Ketkar, and Joseph Tarnowski17 May 2021 | Human Gene Therapy, Vol. 32, No. 9-10Characterization of 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April 2020 | Applied Microbiology and Biotechnology, Vol. 104, No. 11Assessment and streamlined preparation of low-cytotoxicity lentiviral vectors for mobilized human hematopoietic stem cell transductionExperimental Hematology, Vol. 86Production of Lentiviral Vectors Using Suspension Cells Grown in Serum-free MediaMolecular Therapy - Methods & Clinical Development, Vol. 17Current challenges in biotherapeutic particles manufacturing27 November 2019 | Expert Opinion on Biological Therapy, Vol. 20, No. 5Benchmarking of Scale-X Bioreactor System in Lentiviral and Adenoviral Vector Production Hanna M. Leinonen, Saana Lepola, Eevi M. Lipponen, Tommi Heikura, Tiina Koponen, Nigel Parker, Seppo Ylä-Herttuala, and Hanna P. Lesch17 March 2020 | Human Gene Therapy, Vol. 31, No. 5-6Lentiviral Vector Production in Suspension Culture Using Serum-Free Medium for the Transduction of CAR-T Cells10 November 2019Gene therapyImpact of dextran sulfate in culture media on titration of vesicular stomatitis virusJournal of Virological Methods, Vol. 275Titration methods for rVSV-based vaccine manufacturingMethodsX, Vol. 7Establishment of stable Vero cell lines expressing TMPRSS2 and MSPL: A useful tool for propagating porcine epidemic diarrhea virus in the absence of exogenous trypsin29 May 2020 | Virulence, Vol. 11, No. 1Pharmaceutics, Vol. 12, No. 6Lentiviral Vector Purification Using Nanofiber Ion-Exchange ChromatographyMolecular Therapy - Methods & Clinical Development, Vol. 15Identification of novel breakpoints for locus- and region-specific translocations in 293 cells by molecular cytogenetics before and after irradiation22 July 2019 | Scientific Reports, Vol. 9, No. 1Influenza A virus production in a single-use orbital shaken bioreactor with ATF or TFF perfusion systemsVaccine, Vol. 37, No. 47Gene Therapy: Principles and Clinical PotentialProduction of rVSV-ZEBOV in serum-free suspension culture of HEK 293SF cellsVaccine, Vol. 37, No. 44Lentiviral Vectors Come of Age? Hurdles and Challenges in Scaling Up Manufacture19 June 2019Transient Lentiviral Vector Production Using a Packed-Bed Bioreactor System Alexandra McCarron, Martin Donnelley, Chantelle McIntyre, and David Parsons13 June 2019 | Human Gene Therapy Methods, Vol. 30, No. 3RNA Viruses as Tools in Gene Therapy and Vaccine Development1 March 2019 | Genes, Vol. 10, No. 3Optimization of production of vesicular stomatitis virus (VSV) in suspension serum-free culture medium at high cell densityJournal of Biotechnology, Vol. 289New technologies in gene therapy for inducing immune tolerance in hemophilia A3 November 2018 | Expert Review of Clinical Immunology, Vol. 14, No. 12Continuous manufacturing of viral particlesCurrent Opinion in Chemical Engineering, Vol. 22 Volume 28Issue 6Dec 2017 InformationCopyright 2017, Mary Ann Liebert, Inc.To cite this article:Aziza P. Manceur, Howard Kim, Vanja Misic, Nadejda Andreev, July Dorion-Thibaudeau, Stéphane Lanthier, Alice Bernier, Sonia Tremblay, Anne-Marie Gélinas, Sophie Broussau, Rénald Gilbert, and Sven Ansorge.Scalable Lentiviral Vector Production Using Stable HEK293SF Producer Cell Lines.Human Gene Therapy Methods.Dec 2017.330-339.http://doi.org/10.1089/hgtb.2017.086creative commons licensePublished in Volume: 28 Issue 6: December 1, 2017Online Ahead of Print:November 21, 2017Online Ahead of Editing: August 21, 2017Keywordslentiviral vectorstable producerHEK293SFperfusionbioprocessingPDF download

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