Polyethylenimine, a cost‐effective transfection reagent

Abstract In search for a cheap and effective transfection reagent we used the positively charged polyplex‐forming compound polyethylenimine (PEI). This compound is commercially available from different companies either as a non‐modified chemical reagent or with additives as a more cost intensive transfection reagent. Here we used the non‐modified PEI reagent to optimize transfection protocols for different cell‐lines. With these optimized conditions we were able to transiently transfect a number of cell‐lines up to 40–90%.