卵裂球
胞质分裂
线粒体DNA
胚胎
生物
线粒体
劈理(地质)
卵母细胞
细胞生物学
细胞质
细胞分裂
胚泡
遗传学
细胞
胚胎发生
基因
古生物学
断裂(地质)
作者
Yoshiaki Kameyama,Hidehisa Ohnishi,Gaku Shimoi,Ryoichi Hashizume,Masao Ito,Lawrence C. Smith
摘要
A recent report showed higher oxygen consumption, adenosine triphosphate (ATP) production and mitochondrial localisation in trophectoderm cells than in the inner cell mass of mouse blastocysts. We hypothesised that this phenomenon was due to the asymmetrical distribution of mitochondria in the blastomeres during the earlier stages. Oocytes, 2-cell embryos and 4-cell embryos were analysed to determine the volume, ATP content and mitochondrial DNA (mtDNA) copy number in the whole egg and individual blastomeres. Significant differences were detected in the volumes of cytoplasm and ATP contents between blastomeres from the 2-cell and 4-cell embryos. Moreover, whilst remaining stable in whole embryos, mtDNA copy number differed between blastomeres, indicating that mitochondria in oocytes are unevenly delivered into the daughter blastomeres during the first two cleavages. Although their volume and ATP content were not correlated, there was a significant correlation between volume and mtDNA copy number in 2- and 4-cell blastomeres. These results indicate that the number of mitochondria delivered to blastomeres during early cleavage is not precisely equal, suggesting that the allocation of mitochondria into daughter blastomeres is affected by uneven cytoplasmic distribution during cytokinesis in the oocyte and mother blastomeres.
科研通智能强力驱动
Strongly Powered by AbleSci AI