Protective Role of Autophagy in Palmitate-Induced INS-1 β-Cell Death

自噬 ATG5型 程序性细胞死亡 细胞生物学 自噬体 巴非霉素 溶酶体 生物 胃蛋白酶抑制剂 化学 细胞凋亡 生物化学 蛋白酶
作者
Sung-E Choi,Sung-Mi Lee,Youn-Jung Lee,Ling-Ji Li,Soo‐Jin Lee,Ji‐Hyun Lee,Youngsoo Kim,Hee‐Sook Jun,Kwan Woo Lee,Yup Kang
出处
期刊:Endocrinology [Oxford University Press]
卷期号:150 (1): 126-134 被引量:184
标识
DOI:10.1210/en.2008-0483
摘要

Autophagy, a vacuolar degradative pathway, constitutes a stress adaptation that avoids cell death or elicits the alternative cell-death pathway. This study was undertaken to determine whether autophagy is activated in palmitate (PA)-treated β-cells and, if activated, what the role of autophagy is in the PA-induced β-cell death. The enhanced formation of autophagosomes and autolysosomes was observed by exposure of INS-1 β-cells to 400 μm PA in the presence of 25 mm glucose for 12 h. The formation of green fluorescent protein-LC3-labeled structures (green fluorescent protein-LC3 dots), with the conversion from LC3-I to LC3-II, was also distinct in the PA-treated cells. The phospho-mammalian target of rapamycin level, a typical signal pathway that inhibits activation of autophagy, was gradually decreased by PA treatment. Blockage of the mammalian target of rapamycin signaling pathway by treatment with rapamycin augmented the formation of autophagosomes but reduced PA-induced INS-1 cell death. In contrast, reduction of autophagosome formation by knocking down the ATG5, inhibition of fusion between autophagosome and lysosome by treatment with bafilomycin A1, or inhibition of proteolytic degradation by treatment with E64d/pepstatin A, significantly augmented PA-induced INS-1 cell death. These findings showed that the autophagy system could be activated in PA-treated INS-1 β-cells, and suggested that the induction of autophagy might play an adaptive and protective role in PA-induced cell death. Autophagy is activated in palmitate-treated insulinoma-1 beta cells, and the induction of autophagy plays a protective role in palmitate-induced beta cell death.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
nk发布了新的文献求助10
刚刚
科研通AI2S应助慕慕采纳,获得10
1秒前
2秒前
深情安青应助Axin采纳,获得10
2秒前
3秒前
小二郎应助一只羊采纳,获得10
4秒前
小二郎应助一吃一大碗采纳,获得10
4秒前
5秒前
南山鹤完成签到,获得积分10
6秒前
万能图书馆应助I Think采纳,获得10
6秒前
bkagyin应助Hollow采纳,获得10
7秒前
高温预警完成签到,获得积分10
8秒前
解语花发布了新的文献求助30
8秒前
9秒前
10秒前
10秒前
猪嗝铁铁完成签到,获得积分10
11秒前
九亿完成签到,获得积分10
11秒前
Lucas应助寸光采纳,获得10
11秒前
在水一方应助寸光采纳,获得30
11秒前
张鑫发布了新的文献求助10
11秒前
11秒前
11秒前
没问题发布了新的文献求助10
14秒前
木鸽子发布了新的文献求助10
15秒前
yaro发布了新的文献求助10
16秒前
wyp发布了新的文献求助10
16秒前
18秒前
18秒前
asd1576562308完成签到 ,获得积分10
19秒前
20秒前
23秒前
西一阿铭发布了新的文献求助10
24秒前
24秒前
24秒前
LL发布了新的文献求助10
26秒前
26秒前
28秒前
小奕应助宝贝爱吃橙子采纳,获得10
28秒前
健忘捕发布了新的文献求助10
29秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
Immigrant Incorporation in East Asian Democracies 600
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3967482
求助须知:如何正确求助?哪些是违规求助? 3512759
关于积分的说明 11164944
捐赠科研通 3247740
什么是DOI,文献DOI怎么找? 1794021
邀请新用户注册赠送积分活动 874785
科研通“疑难数据库(出版商)”最低求助积分说明 804517