内科学
内分泌学
糖原
胰岛素
骨骼肌
葡萄糖钳夹技术
葡萄糖摄取
刺激
糖酵解
糖原合酶
碳水化合物代谢
氧化磷酸化
生物
化学
胰腺激素
新陈代谢
胰岛素抵抗
生物化学
医学
作者
David E. James,Arthur Jenkins,Edward W. Kraegen
出处
期刊:American Journal of Physiology-endocrinology and Metabolism
[American Physiological Society]
日期:1985-05-01
卷期号:248 (5): E567-E574
被引量:247
标识
DOI:10.1152/ajpendo.1985.248.5.e567
摘要
The euglycemic hyperinsulinemic clamp technique in conscious unrestrained rats was used to examine the effect of insulin on glucose metabolism in metabolically distinct skeletal muscle in vivo. Tissue glucose metabolic rate (R'g) was estimated using 2-[3H]-deoxyglucose, and glucose disposal was examined by measuring glycogen content and [14C]glucose incorporation into glycogen in four different muscles. Insulin sensitivity varied among different muscle types in that the insulin concentration required for half-maximal stimulation of R'g was 80, 150, 280, and 320 mU/1 for soleus (SOL), red gastrocnemius (RG), white gastrocnemius (WG), and extensor digitorum longus, respectively. There were similar relative differences in the maximal effect of insulin on R'g in these muscles. Maximal insulin stimulation almost doubled muscle glycogen content in RG and SOL, whereas there was no change in WG. The relationship between R'g and glycogen synthesis indicated that increased glucose uptake resulted predominantly in glycogen storage. There was an excellent relationship between maximal R'g and blood flow in different muscles. We conclude that there is marked heterogeneity in insulin sensitivity and responsiveness among muscles of different fiber composition. Insulin-induced increases in total peripheral glucose disposal occur predominantly in muscles containing a high proportion of oxidative fibers. Therefore the relative proportion of oxidative to glycolytic muscle fibers may be important factors in determining whole body insulin sensitivity.
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