免疫沉淀
共价键
化学
基质(水族馆)
单克隆抗体
鉴定(生物学)
结合
内生
标志(线性代数)
计算生物学
组合化学
抗体
生物化学
基因
生物
有机化学
遗传学
数学
数学分析
域代数上的
植物
纯数学
生态学
作者
Annette Aichem,Marcus Groettrup
出处
期刊:Methods in molecular biology
日期:2012-01-01
卷期号:: 125-132
被引量:9
标识
DOI:10.1007/978-1-61779-474-2_7
摘要
FAT10 plays a role in many cellular processes. Nevertheless, only one substrate could be identified so far to which FAT10 becomes covalently attached via a nonreducible isopeptide bond. The identification of additional substrates as well as interaction partners is therefore of great interest. Due to the absence of potent anti-FAT10 antibodies, the detection and identification of interaction partners was hindered so far. We have recently described the generation of a novel monoclonal FAT10 antibody that is suitable for immunoprecipitation of endogenous FAT10 and describe in this chapter the detection of endogenous as well as of His-3×FLAG-tagged FAT10 interacting proteins and conjugates by immunoprecipitation using either this novel anti-FAT10 antibody 4FI or an anti-FLAG affinity gel.
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