Investigation of the effect of anticoagulants on platelet recovery, enrichment factor, PDGF, TGF-β1 and optimal dose in the preparation of platelet-rich plasma

柠檬酸钠 血小板 化学 血小板活化 内科学 内分泌学 富血小板血浆 静脉血 血小板源性生长因子受体 生长因子 生物化学 医学 受体 有机化学 病理
作者
Chao Chen,Jun Lei,Xinming Yi,Y.-J. Hua,Jun Yang
出处
期刊:Research Square - Research Square
标识
DOI:10.21203/rs.3.rs-3138006/v1
摘要

Abstract Objective To explore the effect of anticoagulant on platelet recovery and enrichment coefficient of platelet-rich plasma and the optimal dose. Methods Nine New Zealand rabbits were divided into 9 groups with 1 rabbit in each group. Platelet recovery rate, enrichment coefficient, platelet-derived factor (PDGF), transforming growth factor (TGF-β1) contents were observed. Results The enrichment coefficient and platelet recovery of conventional blood + 120% sodium citrate group were the highest, the high dose blood + 120% sodium citrate group was the lowest, the conventional blood + 120% sodium citrate group was the lowest, and the low dose blood + 80% sodium citrate group was the most. Before plasma activation, the expressions of PDGF and TGF-β1 were significantly increased in the other groups compared with the control group, and were higher in the high-concentration blood group. Compared with before activation, TGF-β1 was significantly decreased in the low concentration blood group after activation; TGF-β1 was significantly decreased in the high concentration blood + 80% sodium citrate group; PDGF showed a downward trend in the high concentration blood group; the expression of PDGF was significantly decreased in each group after activation; TGF-β1 was significantly increased in the conventional blood + 120% sodium citrate group. TGF-β1 was significantly decreased in patients treated with low concentration of 80%, 100%, 120% sodium citrate and high concentration of blood + 80% sodium citrate. Conclusion Anticoagulant 120% sodium citrate in conventional blood could separate more platelets, and the enrichment coefficient and platelet recovery rate were the highest, and the biological function was good.
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