硝酸盐
拟南芥
转录因子
抄写(语言学)
细胞内
生物化学
生物
化学
细胞生物学
基因
突变体
生态学
语言学
哲学
作者
Kun-Hsiang Liu,Menghong Liu,Ziwei Lin,Zifu Wang,Binqing Chen,Cong Liu,Aping Guo,Mineko Konishi,Shuichi Yanagisawa,Gerhard Wagner,Jen Sheen
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2022-09-23
卷期号:377 (6613): 1419-1425
被引量:77
标识
DOI:10.1126/science.add1104
摘要
Nitrate is an essential nutrient and signaling molecule for plant growth. Plants sense intracellular nitrate to adjust their metabolic and growth responses. Here we identify the primary nitrate sensor in plants. We found that mutation of all seven Arabidopsis NIN-like protein (NLP) transcription factors abolished plants' primary nitrate responses and developmental programs. Analyses of NIN-NLP7 chimeras and nitrate binding revealed that NLP7 is derepressed upon nitrate perception via its amino terminus. A genetically encoded fluorescent split biosensor, mCitrine-NLP7, enabled visualization of single-cell nitrate dynamics in planta. The nitrate sensor domain of NLP7 resembles the bacterial nitrate sensor NreA. Substitutions of conserved residues in the ligand-binding pocket impaired the ability of nitrate-triggered NLP7 to control transcription, transport, metabolism, development, and biomass. We propose that NLP7 represents a nitrate sensor in land plants.
科研通智能强力驱动
Strongly Powered by AbleSci AI