底漆(化妆品)
麦胚凝集素
互补DNA
化学
DNA
分子生物学
纳米结构
病菌
纳米技术
生物
生物化学
凝集素
微生物学
材料科学
基因
有机化学
作者
Yunju Xiao,Shihua Luo,Jiuxiang Qiu,Ye Zhang,Weijiang Liu,Yunhu Zhao,Yitong Zhu,Yangxi Deng,Mengdi Lu,Suling Liu,Yong Lin,Aiwei Huang,Wen Wang,Xuejiao Hu,Bing Gu
标识
DOI:10.1186/s12951-024-02339-1
摘要
Abstract The capacity to identify small amounts of pathogens in real samples is extremely useful. Herein, we proposed a sensitive platform for detecting pathogens using cyclic DNA nanostructure@AuNP tags (CDNA) and a cascade primer exchange reaction (cPER). This platform employs wheat germ agglutinin-modified Fe 3 O 4 @Au magnetic nanoparticles (WMRs) to bind the E. coli O157:H7, and then triggers the cPER to generate branched DNA products for CDNA tag hybridization with high stability and amplified SERS signals. It can identify target pathogens as low as 1.91 CFU/mL and discriminate E. coli O157:H7 in complex samples such as water, milk, and serum, demonstrating comparable or greater sensitivity and accuracy than traditional qPCR. Moreover, the developed platform can detect low levels of E. coli O157:H7 in mouse serum, allowing the discrimination of mice with early-stage infection. Thus, this platform holds promise for food analysis and early infection diagnosis.
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