组蛋白H1
生物
组蛋白H2A
染色体早凝
遗传学
染色质
组蛋白
组蛋白甲基转移酶
核小体
前期
组蛋白密码
组蛋白甲基化
康德星
细胞生物学
分子生物学
基因
粘蛋白
减数分裂
DNA甲基化
基因表达
作者
Anna Rogers,Nola Neri,Lorencia Chigweshe,Scott G. Holmes
出处
期刊:Genetics
[Oxford University Press]
日期:2024-02-14
卷期号:226 (4)
标识
DOI:10.1093/genetics/iyae022
摘要
Abstract Chromosome condensation is essential for the fidelity of chromosome segregation during mitosis and meiosis. Condensation is associated both with local changes in nucleosome structure and larger-scale alterations in chromosome topology mediated by the condensin complex. We examined the influence of linker histone H1 and variant histone H2A.Z on chromosome condensation in budding yeast cells. Linker histone H1 has been implicated in local and global compaction of chromatin in multiple eukaryotes, but we observe normal condensation of the rDNA locus in yeast strains lacking H1. However, deletion of the yeast HTZ1 gene, coding for variant histone H2A.Z, causes a significant defect in rDNA condensation. Loss of H2A.Z does not change condensin association with the rDNA locus or significantly affect condensin mRNA levels. Prior studies reported that several phenotypes caused by loss of H2A.Z are suppressed by eliminating Swr1, a key component of the SWR complex that deposits H2A.Z in chromatin. We observe that an htz1Δ swr1Δ strain has near-normal rDNA condensation. Unexpectedly, we find that elimination of the linker histone H1 can also suppress the rDNA condensation defect of htz1Δ strains. Our experiments demonstrate that histone H2A.Z promotes chromosome condensation, in part by counteracting activities of histone H1 and the SWR complex.
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