High-performance molecularly imprinted polymers grafted magnetic photonic crystal microspheres for selective enrichment of ochratoxin a

Development of selective enrichment materials for the accurate analysis of ochratoxin a (OTA) in environmental and food samples is an effective way to protect human health. Here, a molecularly imprinted polymer (MIP) known as plastic antibody was synthesized onto the magnetic inverse opal photonic crystal microsphere (MIPCM) using a low-cost dummy template imprinting strategy targeting OTA. The MIP@MIPCM exhibited ultrahigh selectivity with an imprinting factor of 130, high specificity with cross-reactivity factors of 3.3-10.5, and large adsorption capacity of 60.5 μg/mg. Such MIP@MIPCM was used for selective capture of OTA in real samples which was quantified in combination with high-performance liquid chromatography, giving a wide linear detection range of 5-20,000 ng/mL, a detection limit of 0.675 ng/mL, and good recovery rates of 84-116%. Moreover, the MIP@MIPCM can be produced simply and rapidly and is very stable under different environmental conditions and easy to store and transport, so it is an ideal substitute of biological antibody modified materials for the selective enrichment of OTA in real samples.