适体
水溶液中的金属离子
化学
检出限
离子
荧光
Mercury(编程语言)
选择性
费斯特共振能量转移
金属
试剂
自来水
分析化学(期刊)
无机化学
色谱法
生物化学
遗传学
量子力学
环境工程
计算机科学
工程类
生物
程序设计语言
催化作用
物理
有机化学
物理化学
作者
Wei‐Hao Huang,Van-Phung Mai,Ruo-Yin Wu,Ko-Li Yeh,Ruey‐Jen Yang
出处
期刊:Micromachines
[MDPI AG]
日期:2021-10-21
卷期号:12 (11): 1283-1283
被引量:24
摘要
Heavy metal contaminants have serious consequences for the environment and human health. Consequently, effective methods for detecting their presence, particularly in water and food, are urgently required. Accordingly, the present study proposes a sensor capable of detecting mercury Hg(II) and lead Pb(II) ions simultaneously, using graphene oxide (GO) as a quenching agent and an aptamer solution as a reagent. In the proposed device, the aptamer sequences are labeled by FAM and HEX fluorescent dyes, respectively, and are mixed well with 500 ppm GO solution before injection into one inlet of the microchannel, and the heavy metal sample solution is injected into another inlet. The presence of Hg(II) and Pb(II) ions is then detected by measuring the change in the fluorescence intensity of the GO/aptamer suspension as the aptamer molecules undergo fluorescence resonance energy transfer (FRET). The selectivity of these two ions is also shown to be clear among other mixed heavy metal ions. The experimental results show that the aptamer sensors have a linear range of 10~250 nM (i.e., 2.0~50 ppb) for Hg(II) ions and 10~100 nM (i.e., 2.1~20.7 ppb) for Pb(II) ions. Furthermore, the limit of detection is around 0.70 ppb and 0.53 ppb for Hg(II) and Pb(II), respectively, which is lower than the maximum limits of 6 ppb and 10 ppb prescribed by the World Health Organization (WHO) for Hg(II) and Pb(II) in drinking water, respectively.
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