化学
体内
荧光
肝癌
药品
癌症
临床前影像学
肝病
生物物理学
癌症研究
生物化学
药理学
内科学
生物
物理
生物技术
医学
量子力学
作者
Yuexia Yang,Liang Zhang,Jiajun Li,Jiemin Wang,Ting Cao,Lei Zheng,Yuping Cao,Wenwu Qin,Yun Liu
标识
DOI:10.1016/j.aca.2021.338621
摘要
Leucine aminopeptidase (LAP) as an important proteolytic enzyme, has been mainly found in hepatobiliary cells, and overexpressed in hepatoma cells. Herein, a new highly selective red-emitting fluorescent probe (DCDHF-Ala) for LAP has been synthesized based on 2-dicyanomethyldiene-3-cyano-2, 5-dihydrofuran (DCDHF) as fluorophore, and alanine (Ala) as the detection group. More importantly, it's the first time to use Ala as a reactive group for LAP. DCDHF-Ala has a low detection limit (0.20 U/L), excellent water solubility and cell membrane permeability. In addition, the probe has been successfully applied to fluorescent imaging in cells and zebrafish. It's especially worth mentioning that, DCDHF-Ala has a high biosafety and enables a real-time detection of LAP levels in mice model. What's the most important is that DCDHF-Ala may be an effective tool to qualitatively monitor the upregulation of LAP induced by liver injury and liver cancer.
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