光遗传学
神经科学
体感系统
生物
电生理学
感觉系统
细胞神经科学
沟道视紫红质
生物神经网络
转基因生物
绿色荧光蛋白
荧光蛋白
基因
遗传学
作者
Walther Akemann,Hiroki Mutoh,Amélie Perron,Jean Rossier,Thomas Knöpfel
出处
期刊:Nature Methods
[Springer Nature]
日期:2010-07-11
卷期号:7 (8): 643-649
被引量:262
摘要
Cortical information processing relies on synaptic interactions between diverse classes of neurons with distinct electrophysiological and connection properties. Uncovering the operational principles of these elaborate circuits requires the probing of electrical activity from selected populations of defined neurons. Here we show that genetically encoded voltage-sensitive fluorescent proteins (VSFPs) provide an optical voltage report from targeted neurons in culture, acute brain slices and living mice. By expressing VSFPs in pyramidal cells of mouse somatosensory cortex, we also demonstrate that these probes can report cortical electrical responses to single sensory stimuli in vivo. These protein-based voltage probes will facilitate the analysis of cortical circuits in genetically defined cell populations and are hence a valuable addition to the optogenetic toolbox.
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