脱氧核酶
血红素
化学
G-四倍体
寡核苷酸
组合化学
检出限
过氧化物酶
比色法
DNA
过氧化氢
生物传感器
辣根过氧化物酶
催化作用
基质(水族馆)
显色的
色谱法
试剂
肉眼
生物化学
酶
血红素
地质学
海洋学
作者
Bingling Li,Yan Du,Tao Li
标识
DOI:10.1016/j.aca.2009.09.009
摘要
In this work, the suitability of 3,3',5,5'-tetramethylbenzidine sulfate (TMB) as the substrate of a DNAzyme catalytic system composed of a guanine-quadruplex DNA molecule and hemin was investigated. In the presence of H2O2, the hemin-DNA complex catalyzes the oxidation of TMB to produce two colored products, much like a peroxidase. The color-generating activity of this system could be influenced by several factors such as buffer type, pH value, DNA sequence, reaction time, and concentrations of both the hemin and H2O2. To illustrate the utility of this catalytic system, we designed a colorimetric assay, in which a synthetic oligonucleotide with a sequence complementary to the G-quadruplex DNA was used as the target. A detection limit of 1.86 nM was obtained. Our data have shown that TMB was an excellent colorimetric indicator that reported the peroxidase activities of the widely studied hemin-G-quadruplex DNAzyme system.
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