已入深夜,您辛苦了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!祝你早点完成任务,早点休息,好梦!

Relevance of Ara h1, Ara h2 and Ara h3 in peanut‐allergic patients, as determined by immunoglobulin E Western blotting, basophil–histamine release and intracutaneous testing: Ara h2 is the most important peanut allergen

嗜碱性粒细胞 免疫球蛋白E 组胺 过敏原 免疫学 连续稀释 离体 嗜碱性粒细胞活化 医学 过敏 体内 抗体 药理学 生物 生物技术 病理 替代医学
作者
Stef J. Koppelman,Michel Wensing,M. Ertmann,André C. Knulst,Edward F. Knol
出处
期刊:Clinical & Experimental Allergy [Wiley]
卷期号:34 (4): 583-590 被引量:255
标识
DOI:10.1111/j.1365-2222.2004.1923.x
摘要

A number of allergenic proteins in peanut has been described and the relative importance of these allergens is yet to be determined.We have investigated the relevance of previously identified peanut allergens in well-characterized peanut-allergic patients by in vitro, ex vivo and in vivo assays.Thirty-two adult peanut-allergic patients were included based on careful and standardized patient history and the presence of peanut-specific IgE. The diagnosis peanut allergy was confirmed using double-blind placebo-controlled food challenges in 23 patients. Major peanut allergens Ara h1, Ara h2 and Ara h3 were purified from peanuts using ion-exchange chromatography. IgE immunoblotting was performed and IgE-cross-linking capacity was examined by measuring histamine release (HR) after incubating patient basophils as well as passively sensitized basophils with several dilutions of the allergens. Intracutaneous tests (ICTs) using 10-fold dilution steps of the purified allergens and crude peanut extract were performed.Ara h2 was recognized most frequently (26 out of 32) in all tests and induced both positive skin tests and basophil degranulation at low concentrations, whereas Ara h1 and Ara h3 were recognized less frequently and reacted only at 100-fold higher concentrations as analysed with HR and intracutaneous testing (ICT). Next to the three tested allergens, proteins with molecular weights of somewhat smaller than 15 kDa were identified as a IgE-binding proteins on immunoblot in the majority of the patients (20 out of 32).We conclude that Ara h2 is, for our patient group, the most important peanut allergen, and that previously unidentified peanut proteins with molecular weights of somewhat smaller than 15 kDa may be important allergens as well. ICT in combination with basophil-HR and IgE immunoblotting provides insight in the patient specificity towards the individual peanut allergens.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
oceanao应助sukasuka采纳,获得10
2秒前
3秒前
安静的棉花糖完成签到 ,获得积分10
3秒前
caia完成签到,获得积分10
3秒前
5秒前
6秒前
6秒前
丁丁猫老大完成签到 ,获得积分20
6秒前
7秒前
8秒前
田様应助lurongjun采纳,获得10
8秒前
阿东c完成签到 ,获得积分10
10秒前
10秒前
caia发布了新的文献求助10
10秒前
shuhaha发布了新的文献求助10
11秒前
11秒前
所所应助背后的幻巧采纳,获得10
12秒前
whereas完成签到 ,获得积分10
15秒前
寒武纪完成签到,获得积分10
16秒前
xx完成签到 ,获得积分10
19秒前
20秒前
卓诗云发布了新的文献求助10
23秒前
Tony完成签到,获得积分10
23秒前
寒武纪关注了科研通微信公众号
25秒前
今后应助丁丁猫老大采纳,获得10
26秒前
顾矜应助丁丁猫老大采纳,获得10
26秒前
hky完成签到 ,获得积分10
28秒前
duxh123完成签到 ,获得积分10
29秒前
30秒前
31秒前
难过的人生完成签到 ,获得积分10
31秒前
123关闭了123文献求助
34秒前
35秒前
TBLS发布了新的文献求助10
37秒前
oceanao应助科研菜鸟采纳,获得10
37秒前
华仔应助结实的德地采纳,获得10
38秒前
动听靖完成签到 ,获得积分10
39秒前
Mrshi发布了新的文献求助10
39秒前
yinan完成签到,获得积分10
39秒前
yalon关注了科研通微信公众号
40秒前
高分求助中
Evolution 10000
юрские динозавры восточного забайкалья 800
English Wealden Fossils 700
Distribution Dependent Stochastic Differential Equations 500
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
A new species of Velataspis (Hemiptera Coccoidea Diaspididae) from tea in Assam 500
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3158476
求助须知:如何正确求助?哪些是违规求助? 2809636
关于积分的说明 7883043
捐赠科研通 2468315
什么是DOI,文献DOI怎么找? 1314077
科研通“疑难数据库(出版商)”最低求助积分说明 630572
版权声明 601956