Qishen granules exerts cardioprotective effects on rats with heart failure via regulating fatty acid and glucose metabolism

心力衰竭 碳水化合物代谢 新陈代谢 内科学 药理学 脂肪酸 化学 脂肪酸代谢 传统医学 生物化学 医学
作者
Kuo Gao,Jian Zhang,Pengrong Gao,Qiyan Wang,Ying Liu,Junjie Liu,Yili Zhang,Yan Li,Hong Chang,Ping Ren,Jinmin Liu,Yong Wang,Wei Wang
出处
期刊:Chinese Medicine [Springer Nature]
卷期号:15 (1) 被引量:24
标识
DOI:10.1186/s13020-020-0299-9
摘要

Abstract Background Qishen granules (QSG) has been applied to treat heart failure (HF) for decades. Our previous transcriptomics study has suggested that Qishen granules (QSG) could regulate the pathways of cardiac energy metabolism in HF, but the specific regulatory mechanism has not yet been clarified. This study was to investigate the potential mechanism of QSG in regulating myocardial fatty acid (FA) and glucose metabolism in a rat model of HF. Methods The model of HF was induced by left anterior descending coronary artery ligation. Cardiac structure and function were assessed by cine magnetic resonance imaging (MRI) and echocardiography. Level of glucose metabolism was non-invasively evaluated by 18 F-fluorodeoxyglucose positron emission tomography/computed tomography (PET/CT). Blood lipid levels were determined by enzymatic analysis. The mitochondrial ultrastructure was observed with a transmission electron microscope. The critical proteins related to FA metabolism, glucose metabolism and mitochondrial function were measured by western blotting. The ANOVA followed by a Fisher’s LSD test was used for within-group comparisons. Results QSG ameliorated cardiac functions and attenuated myocardial remodeling in HF model. The levels of serum TC, TG and LDL-C were significantly reduced by QSG. The proteins mediating FA uptake, transportation into mitochondria and β-oxidation (FAT/CD36, CPT1A, ACADL, ACADM, ACAA2 and SCP2) as well as the upstreaming transcriptional regulators of FA metabolism (PPARα, RXRα, RXRβ and RXRγ) were up-regulated by QSG. As to glucose metabolism, QSG inhibited glycolytic activity by decreasing LDHA, while stimulated glucose oxidation by decreasing PDK4. Furthermore, QSG could facilitate tricarboxylic acid cycle, promote the transportation of ATP from mitochondria to cytoplasm and restore the mitochondrial function by increasing SUCLA2, CKMT2 and PGC-1α and decreasing UCP2 simultaneously. Conclusion QSG improved myocardial energy metabolism through increasing FA metabolism,inhibiting uncoupling of glycolysis from glucose oxidation.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
HR112应助uh采纳,获得20
3秒前
奋斗的杰发布了新的文献求助10
4秒前
林lin发布了新的文献求助10
7秒前
幸福大白发布了新的文献求助10
9秒前
12544593556发布了新的文献求助10
10秒前
万能图书馆应助study采纳,获得10
10秒前
14秒前
ShowMaker应助机械师简采纳,获得30
14秒前
15秒前
Hello应助Need_Knowledge采纳,获得10
17秒前
Ning发布了新的文献求助10
18秒前
QMCL完成签到,获得积分10
19秒前
小小学神发布了新的文献求助10
20秒前
林lin完成签到,获得积分10
21秒前
佳佳完成签到,获得积分10
24秒前
25秒前
研友_Lmbz1n完成签到,获得积分10
27秒前
LONG发布了新的文献求助10
28秒前
le完成签到,获得积分10
28秒前
31秒前
binyh完成签到,获得积分10
31秒前
32秒前
34秒前
36秒前
阿rain完成签到,获得积分10
37秒前
roger969发布了新的文献求助30
38秒前
梅槑完成签到 ,获得积分10
39秒前
shain发布了新的文献求助10
39秒前
李爱国应助jitanxiang采纳,获得10
39秒前
充电宝应助12544593556采纳,获得10
41秒前
上官若男应助shain采纳,获得10
45秒前
47秒前
白茶清欢完成签到,获得积分10
47秒前
小巧问芙完成签到 ,获得积分10
49秒前
白茶清欢发布了新的文献求助10
51秒前
orixero应助Cynthia采纳,获得10
51秒前
54秒前
多喝冰水完成签到,获得积分10
54秒前
57秒前
oceanao应助实心球采纳,获得10
59秒前
高分求助中
Evolution 10000
ISSN 2159-8274 EISSN 2159-8290 1000
Becoming: An Introduction to Jung's Concept of Individuation 600
Ore genesis in the Zambian Copperbelt with particular reference to the northern sector of the Chambishi basin 500
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
A new species of Velataspis (Hemiptera Coccoidea Diaspididae) from tea in Assam 500
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3161114
求助须知:如何正确求助?哪些是违规求助? 2812494
关于积分的说明 7895538
捐赠科研通 2471395
什么是DOI,文献DOI怎么找? 1315941
科研通“疑难数据库(出版商)”最低求助积分说明 631074
版权声明 602103