An improved method for the separation of carotenoids and carotenoid isomers by liquid chromatography–mass spectrometry

类胡萝卜素 叶黄素 玉米黄质 化学 色谱法 番茄红素 胡萝卜素 质谱法 有机化学 食品科学
作者
Shuang Li,Xinyi Tang,Yingying Lu,Jilin Xu,Juanjuan Chen,Haimin Chen
出处
期刊:Journal of Separation Science [Wiley]
卷期号:44 (2): 539-548 被引量:21
标识
DOI:10.1002/jssc.202000902
摘要

Abstract Carotenoids consist of a series of conjugated isoprene units that are characteristically highly conjugated through double bonds, leading to the formation of many isomers that are susceptible to oxidation and other chemical modifications. Extreme hydrophobicity and high complexity make carotenoids difficult to identify and quantify. We implemented the use of a common Syncronis C18 column with strong eluting solvent, here isopropanol, to successfully separate a mixture of 23 carotenoids standards with different structural properties. In addition, the method differentiated between three groups of isomeric carotenoids (lycopene/δ‐carotene/γ‐carotene/ε‐carotene/α‐carotene/β‐carotene, α‐cryptoxanthin/β‐cryptoxanthin, and zeaxanthin/lutein) by optimizing the gradient profile and using liquid chrmatography–mass spectrometry. The LOD ranged from 0.05 to 5.51 ng/mL, and the recovery of carotenoids in Mytilus coruscus was from 63.54 to 93.25%, with standard deviations <10%. Twenty‐five carotenoids were detected with a total content of 857 ± 55.1 mg/kg, and three isomeric carotenoids were identified: ε‐carotene, α‐carotene, and β‐carotene. Our results show that this methodology is a significant improvement over other alternatives for analyzing carotenoids because of its compatibility with carotenoids of different categories, and most importantly, its ability to resolve isomeric carotenes, which is significant not only for assessing carotenoid species, but also for the tracing of metabolic pathways of carotenoids.
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