生物
造血
缺氧(环境)
细胞生物学
小RNA
细胞分化
脐带血
红细胞生成
异位表达
川地34
转染
干细胞
分子生物学
基因
免疫学
遗传学
内科学
化学
医学
有机化学
氧气
贫血
作者
Xue Shi,Hua Wang,Fanxuan Kong,Qinqin Xu,Fengjun Xiao,Yuefeng Yang,Ri‐Li Ge,Lisheng Wang
标识
DOI:10.1016/j.yexcr.2016.12.023
摘要
MicroRNAs (miRNAs) regulate the hypoxia-induced erythroid differentiation of hematopoietic cells. In this study, we identified that miR-486 was a rapid response miRNA to hypoxia in erythroleukemia TF-1 cells. Hypoxia exposure increased both intracellular and miR-486 levels of TF-1 cells. Ectopic miR-486 expression enhanced the growth and erythroid differentiation of TF-1 cells, whereas miR-486 inhibition suppressed their growth and erythroid differentiation. Treatment of TF-1 and cord blood CD34+ cells with exogenous containing miR-486 resulted in an increase of intracellular miR-486 level and enhanced erythroid differentiation. Furthermore, we identified that Sirt1 is a miR-486 target gene which modulates hypoxia-induced erythroid differentiation of TF-1 cells. Thus we identified a novel miRNA regulatory network that contributes to hypoxia-induced erythroid differentiation of hematopoietic cells.
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