检出限
癌胚抗原
校准曲线
二茂铁
电化学气体传感器
材料科学
电化学
胶体金
线性范围
循环伏安法
色谱法
电解质
伏安法
分析化学(期刊)
化学
纳米技术
电极
纳米颗粒
医学
物理化学
内科学
癌症
作者
Xuefang Gu,Zhe She,Tianxiao Ma,Shu Tian,Heinz‐Bernhard Kraatz
标识
DOI:10.1016/j.bios.2017.12.014
摘要
In this work, a sandwich-type electrochemical immunosensor for carcinoembryonic antigen (CEA) detection has been constructed and tested. Unlike many other sensors using external electrochemical species in the electrolyte to generate an electrochemical signal, a ferrocene derivative has been integrated into the design of the sensor to provide an internal reporting system, allowing detection of CEA in buffers and biological samples. Gold nanoparticles, which have been used to increase the conductivity of sensing surfaces, also carry immobilized secondary anti-CEA and a ferrocene derivative. The shelf life testing of the sensor shows good performance after storage for 4 weeks. The sensor has been calibrated against different concentration of the target protein using square wave voltammetry. The calibration curve has been obtained in the range of 0.05-20ngmL-1, and the detection limit for CEA is ~ 0.01ngmL-1. The capability of the immunosensor has been verified by performing detection of CEA in human serum samples.
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