银纳米粒子
工业发酵
纳米颗粒
萃取(化学)
发酵
生物膜
化学
核化学
基质(化学分析)
细菌
中心组合设计
活性炭
化学工程
材料科学
色谱法
响应面法
纳米技术
食品科学
有机化学
吸附
生物
工程类
遗传学
作者
Arivizhivendhan Kannan Villalan,M. Mahesh,R. Boopathy,Karthikeyan Sekar,Rathanasamy Regina Mary,G. Sekaran
标识
DOI:10.1016/j.apsusc.2017.08.128
摘要
A novel continuous production and extraction of bacterial bioactive prodigiosin (PG) from fermented using silver nanoparticle impregnated functioned activated carbon composite is proposed for cost-effective and ecofriendly microbial technique. Hence, in this investigation silver nanoparticle was impregnated onto functioned activated carbon ([AC]F) as a support matrix and to enable the separation of PG conjugated silver nanoparticle from the fermented medium. A laboratory scale experiment was carried out to evaluate the continuous production and recovery of PG using [[email protected]]F. Ag nanoparticle impregnated [AC]F ([[email protected]]F) characterized by FT-IR, XRD, TGA, DSC and SEM. Instrumental analyses confirmed that Ag nanoparticles significantly impregnated on AC through the functionalization of AC with diethanolamine and it enhances the binding capacity between AC and Ag. The various process parameters, such as contact time, pH, and mass of [[email protected]]F, were statistically optimized for the recovery of PG using Response Surface Methodology (RSM). The maximum extraction of PG in [[email protected]]F was found to be 16.2 ± 0.2 mg g−1, its twofold higher than [AC]F. Further, PG conjugated [[email protected]]F and ([[email protected]]F-PG) were checked for the growth inhibition of gram negative and gram positive bacteria without formation of biofilm upto 96 h. Hence, the developed matrix could be eco-friendly, viable and lower energy consumption step for separation of the bacterial bioactive PG from fermented broth. In additionally, [[email protected]]F-PG was used as an antifouling matrix without formation of biofilm.
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