生物分子
X射线光电子能谱
涂层
表征(材料科学)
材料科学
纳米技术
沉积(地质)
基质(水族馆)
化学工程
图层(电子)
化学
古生物学
工程类
地质学
海洋学
生物
沉积物
作者
Simona Rella,Elisabetta Mazzotta,Antonio Caroli,Maria De Luca,Cecilia Bucci,Cosimino Malitesta
标识
DOI:10.1016/j.apsusc.2018.03.057
摘要
Polydopamine (PDA) films have attracted a rapidly increasing research attention during the last years due to its simple and rapid deposition under alkaline conditions in substrate independent manner providing a universal coating for materials with different chemical and physical properties. Furthermore, this polymerized layer is enriched with functional groups that enable immobilization of primary amine or thiol-based biomolecules via a simple dipping process. Although these aspects justify PDA wide and successful application as a versatile coating for biomolecule immobilization, several aspects have not been deeply investigated leaving some key details unclear and thus limiting PDA practical applications. A number of approaches are commonly used for the growth of PDA, but the effect of deposition conditions on film properties, which in turn influence biomolecule immobilization has not been systematically investigated yet. In the present work, an extensive characterization by X-ray Photoelectron Spectroscopy (XPS) is performed on PDA coatings grown under different experimental conditions. Comparison of XPS data about elemental composition, distribution of functional groups and thickness of PDA coatings provided valuable information for identifying more suitable PDA coating for biomolecule anchoring, further explored by in vitro experiments.
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