核糖核蛋白
基因组编辑
生物
清脆的
素数(序理论)
生殖系
核糖核酸
引导RNA
斑马鱼
计算生物学
细胞生物学
基因
遗传学
分子生物学
组合数学
数学
作者
Karl Petri,Weiting Zhang,Junyan Ma,Andrea Schmidts,Hyunho Lee,Joy E. Horng,Daniel Y. Kim,Ibrahim C. Kurt,Kendell Clement,Jonathan Y. Hsu,Luca Pinello,Marcela V. Maus,J. Keith Joung,Jing-Ruey Joanna Yeh
标识
DOI:10.1038/s41587-021-00901-y
摘要
Prime editors have been delivered using DNA or RNA vectors. Here we demonstrate prime editing with purified ribonucleoprotein complexes. We introduced somatic mutations in zebrafish embryos with frequencies as high as 30% and demonstrate germline transmission. We also observed unintended insertions, deletions and prime editing guide RNA (pegRNA) scaffold incorporations. In HEK293T and primary human T cells, prime editing with purified ribonucleoprotein complexes introduced desired edits with frequencies of up to 21 and 7.5%, respectively.
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