Physicochemical Properties and Skin Protection Activities of Polysaccharides from Usnea longissima by Graded Ethanol Precipitation

Four Usnea longissima polysaccharides (ULPs; ULP15, ULP30, ULP50, and ULP70) were obtained from the lichen U. longissima via water extraction and graded ethanol precipitation. The obtained ULPs were all heteropolysaccharides with a few proteins, with which glucose was the major monosaccharide composition. With the increase in the precipitated ethanol concentrations, the content of galactose, xylose, and mannose increased, whereas that of glucose decreased. Moreover, the antioxidant activity test demonstrated that ULP15 exhibited better reducing power and stronger scavenging ability on 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl free radicals. Importantly, ULP15 also had a better proliferative effect on human HaCaT keratinocytes and dermal fibroblasts. Meanwhile, ULP15 protected HaCaT keratinocytes from UVB-induced proliferation inhibition and exhibited tyrosinase inhibition activity. Therefore, this work provides interesting insight into the preparation of cosmetic ingredients using the polysaccharide ULP15.