RNA-seq is the most rapidly developed biomedical technology in recent years. It is a more accurate determination and analysis method at the transcription level, and has a wide range and important application in the field of passive plant research. In order to measure gene expression more accurately, most scientific research experiments will use RNA-seq technology. First, the RNA-seq technology uses reverse transcription to design a gene library, and then sequencing to screen genes. The process of analyzing the relationship between genes and functions using various analytical techniques. It is widely used by researchers mainly because of its high-throughput technology. This article introduces the application of RNA-seq technology in screening genes at home and abroad and the introduction of the experimental process, and makes a review.